Expressing:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Flow Cytometry:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Bioprocessing:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Control:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Cell Culture:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Staining:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Clone Assay:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Labeling:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Confocal Microscopy:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Incubation:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Co-Culture Assay:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Irradiation:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Enzyme-linked Immunosorbent Assay:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Derivative Assay:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Dot Blot:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Lysis:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
Cytotoxicity Assay:Article Title: Lymphocyte imprinting with melanoma antigens acquired by trogocytosis facilitates identification of tumor-reactive T cells
Article Snippet: APC-conjugated-mouse anti human NG2/MCSP (CSPG4) (R&D Systems, Minneapolis, MN), mouse anti-human HMB45 (Serotec, Oxford, UK), FITC-conjugated mouse anti-human CD146, FITC-conjugated rat anti-human CD8 antibody (Serotec), FITC- or APC-conjugated mouse anti human CD8, PE or FITC conjugated mouse anti-human CD4, PE-conjugated mouse anti human IFN-γ, FITC- conjugated mouse anti-human CD107A (LAMP-1) (all from eBioscience, San Diego, CA), pacific blue-conjugated mouse anti-human CD107 (Biolegend), APC-mouse anti-human CD137 (BD Pharmingen), APC or DyLight 649-conjugated donkey anti-mouse IgG (Jackson, West Grove, PA) were purchased from the indicated companies.
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